BAIT

AR

AIS, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, RP11-383C12.1
androgen receptor
GO Process (23)
GO Function (16)
GO Component (5)
Homo sapiens
PREY

FOXO1

FKH1, FKHR, FOXO1A
forkhead box O1
GO Process (30)
GO Function (6)
GO Component (5)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

AKT-independent protection of prostate cancer cells from apoptosis mediated through complex formation between the androgen receptor and FKHR.

Li P, Lee H, Guo S, Unterman TG, Jenster G, Bai W

Recent studies suggested that the protection of cell apoptosis by AKT involves phosphorylation and inhibition of FKHR and related FOXO forkhead transcription factors and that androgens provide an AKT-independent cell survival signal in prostate cancer cells. Here, we report receptor-dependent repression of FKHR function by androgens in prostate cancer cells. Transcriptional analysis demonstrated that activation of the androgen receptor caused ... [more]

Mol. Cell. Biol. Jan. 01, 2003; 23(1);104-18 [Pubmed: 12482965]

Throughput

  • Low Throughput

Additional Notes

  • Mapping analysis localized the interacting domains to the carboxyl terminus between amino acids 350 and 655 of FKHR and to the amino-terminal A/B region and the ligand binding domain of the receptor

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AR FOXO1
Far Western
Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Low-BioGRID
244613
AR FOXO1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
244612

Curated By

  • BioGRID