BAIT

H2AFX

AW228881, H2A.X, H2ax, Hist5-2ax, gammaH2ax
H2A histone family, member X
Mus musculus
PREY

TP53BP1

53BP1, p202
tumor protein p53 binding protein 1
Homo sapiens

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks.

Celeste A, Fernandez-Capetillo O, Kruhlak MJ, Pilch DR, Staudt DW, Lee A, Bonner RF, Bonner WM, Nussenzweig A

Histone H2AX is rapidly phosphorylated in the chromatin micro-environment surrounding a DNA double-strand break (DSB). Although H2AX deficiency is not detrimental to life, H2AX is required for the accumulation of numerous essential proteins into irradiation induced foci (IRIF). However, the relationship between IRIF formation, H2AX phosphorylation (gamma-H2AX) and the detection of DNA damage is unclear. Here, we show that the ... [more]

Nat. Cell Biol. Jul. 01, 2003; 5(7);675-9 [Pubmed: 12792649]

Throughput

  • Low Throughput

Additional Notes

  • Migration of repair and signalling proteins to DSBs is not abrogated in H2AX(-/-) cells, or in H2AX-deficient cells that have been reconstituted with H2AX mutants that eliminate phosphorylation; despite their initial recruitment to DSBs, numerous factors,

Curated By

  • BioGRID