HRAS
Gene Ontology Biological Process
- Ras protein signal transduction [ISO, TAS]
- apoptotic process [ISO]
- cell aging [IDA]
- cell cycle arrest [ISO]
- cell proliferation [IPI]
- cellular senescence [ISO]
- endocytosis [IDA]
- intrinsic apoptotic signaling pathway [IGI, IMP]
- mitotic cell cycle checkpoint [ISO]
- negative regulation of Rho GTPase activity [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of neuron apoptotic process [IDA, IGI]
- positive regulation of DNA replication [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of JNK cascade [ISO]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of Rac GTPase activity [ISO]
- positive regulation of Ras protein signal transduction [ISO]
- positive regulation of actin cytoskeleton reorganization [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of cell proliferation [IDA, ISO]
- positive regulation of epithelial cell proliferation [ISO]
- positive regulation of gene expression [IDA]
- positive regulation of miRNA metabolic process [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of ruffle assembly [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of wound healing [ISO]
- protein heterooligomerization [ISO]
- regulation of long-term neuronal synaptic plasticity [IMP]
- small GTPase mediated signal transduction [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RAF1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- Ras protein signal transduction [TAS]
- activation of MAPKK activity [IDA, TAS]
- activation of adenylate cyclase activity [NAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell proliferation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- ion transmembrane transport [TAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [TAS]
- negative regulation of protein complex assembly [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- platelet activation [TAS]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- regulation of Rho protein signal transduction [TAS]
- regulation of apoptotic process [TAS]
- regulation of cell differentiation [TAS]
- regulation of cell motility [TAS]
- signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- synaptic transmission [TAS]
- transmembrane transport [TAS]
- wound healing [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
14-3-3 zeta negatively regulates raf-1 activity by interactions with the Raf-1 cysteine-rich domain.
Although Raf-1 is a critical effector of Ras signaling and transformation, the mechanism by which Ras promotes Raf-1 activation is complex and remains poorly understood. We recently reported that Ras interaction with the Raf-1 cysteine-rich domain (Raf-CRD, residues 139-184) may be required for Raf-1 activation. The Raf-CRD is located in the NH2-terminal negative regulatory domain of Raf-1 and is highly ... [more]
Throughput
- Low Throughput
Additional Notes
- Mutation of Raf-1 residues 143-145 impairs binding of 14-3-3, but not Ras, to the Raf-CRD. Introduction of mutations that impair 14-3-3 binding resulted in full-length Raf-1 mutants with enhanced transforming activity
Curated By
- BioGRID