BAIT

CHO2

PEM1, phosphatidylethanolamine N-methyltransferase, L000000328, YGR157W

Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis

GO Process (1)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

phosphatidylcholine biosynthetic process [IDA, IGI, IMP]

Gene Ontology Molecular Function

phosphatidylethanolamine N-methyltransferase activity [IDA, IGI, IMP]

Gene Ontology Cellular Component

endoplasmic reticulum [IDA]

integral component of membrane [ISM]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

LPL1

YOR059C, YOR29-10, putative hydrolase, YOR059C

Phospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Phospholipase Lpl1 links lipid droplet function with quality control protein degradation.

Weisshaar N, Welsch H, Guerra-Moreno A, Hanna J

Protein misfolding is toxic to cells and is believed to underlie many human diseases, including many neurodegenerative diseases. Accordingly, cells have developed stress responses to deal with misfolded proteins. The transcription factor Rpn4 mediates one such response and is best known for regulating the abundance of the proteasome, the complex multisubunit protease that destroys proteins. Here we identify Lpl1 as ... [more]

Mol. Biol. Cell Mar. 15, 2017; 28(6);716-725 [Pubmed: 28100635]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)
phenotype: resistance to chemicals (APO:0000087)

Additional Notes

Figure 4

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CHO2 LPL1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3111	BioGRID	382987
CHO2 LPL1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2733	BioGRID	2121946
LPL1 CHO2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3785	BioGRID	2182898
CHO2 LPL1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Surma MA (2013)	High	-12.6284	BioGRID	899749

Curated By

BioGRID

Interaction Summary