NR3C1
Gene Ontology Biological Process
- cellular response to steroid hormone stimulus [IDA]
- gene expression [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA]
- signal transduction [TAS]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- glucocorticoid receptor activity [TAS]
- glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- steroid binding [IDA]
- steroid hormone binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- glucocorticoid receptor activity [TAS]
- glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- steroid binding [IDA]
- steroid hormone binding [IDA]
Gene Ontology Cellular Component
PRDX3
Gene Ontology Biological Process
- cellular response to oxidative stress [IDA]
- cellular response to reactive oxygen species [IMP]
- hydrogen peroxide catabolic process [IGI, IMP]
- mitochondrion organization [IMP]
- myeloid cell differentiation [ISS]
- negative regulation of apoptotic process [IDA, IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of kinase activity [IDA]
- peptidyl-cysteine oxidation [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of cell proliferation [IDA]
- regulation of mitochondrial membrane potential [IMP]
- response to hydrogen peroxide [IDA]
- response to lipopolysaccharide [ISS]
- response to oxidative stress [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Proteome-scale Binary Interactomics in Human Cells.
Because proteins are the main mediators of most cellular processes they are also prime therapeutic targets. Identifying physical links among proteins and between drugs and their protein targets is essential in order to understand the mechanisms through which both proteins themselves and the molecules they are targeted with act. Thus, there is a strong need for sensitive methods that enable ... [more]
Throughput
- High Throughput
Additional Notes
- Interaction with Glucocorticoid Receptor (GR) was downregulated in the presence of dexamethasone.
- MAPPIT (Mammalian Protein-Protein Interaction Trap)
Curated By
- BioGRID