BAIT

BRCA1

BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
breast cancer 1, early onset
GO Process (44)
GO Function (10)
GO Component (11)

Gene Ontology Biological Process

Homo sapiens

Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Publication

Identification of proteins that interact with BRCA1 by Far-Western library screening.

Yarden RI, Brody LC

Protein-protein interactions control numerous biological processes. In the case of a protein with no known function, identification of interacting proteins may lend insight into its cellular function. Protein-protein interactions are often detected by yeast two-hybrid screening which is based on a transcriptional read-out. One limitation of this technique is that transcription factors, when used as bait, frequently impair the effectiveness ... [more]

J. Cell. Biochem. Dec. 18, 2001; 83(4);521-31 [Pubmed: 11746496]

Throughput

  • Low Throughput

Additional Notes

  • Recombinant histidine-tagged BRCT as the primary protein probe;chimeric clone identical to mitochondrial rRNA 16S gene (GenBank accession no. V00710.1) and wingless-type MMTV-13 (GenBank accession no Z71621)

Curated By

  • BioGRID