BCL2L1
Gene Ontology Biological Process
- apoptotic mitochondrial changes [TAS]
- apoptotic process [TAS]
- cytokinesis [IMP]
- extrinsic apoptotic signaling pathway in absence of ligand [IBA]
- innate immune response [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- mitotic cell cycle checkpoint [IMP]
- negative regulation of anoikis [IMP]
- negative regulation of apoptotic process [IDA, IMP]
- negative regulation of autophagy [TAS]
- negative regulation of establishment of protein localization to plasma membrane [IDA]
- negative regulation of execution phase of apoptosis [IDA]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- negative regulation of intrinsic apoptotic signaling pathway [IDA]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- negative regulation of release of cytochrome c from mitochondria [IC, IDA]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- positive regulation of intrinsic apoptotic signaling pathway [TAS]
- regulation of mitochondrial membrane permeability [IDA]
- regulation of mitochondrial membrane potential [IDA]
- release of cytochrome c from mitochondria [IDA]
- response to cytokine [IDA]
- suppression by virus of host apoptotic process [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HRK
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
High-throughput methods to detect dimerization of Bcl-2 family proteins.
Members of the Bcl-2 family are critical regulators of apoptosis. Antiapoptotic family proteins such as Bcl-2 and Bcl-x(L) function, at least in part, by binding proapoptotic members such as Bax and Bak and thereby preventing release of apoptotic proteins, including cytochrome c, from the mitochondria. "BH3-only" members of the family disrupt this interaction by binding, via their BH3 domain, to ... [more]
Throughput
- Low Throughput
Additional Notes
- Scintillation proximity assay and a time-resolved fluorescence resonance energy transfer assay (HTRF); Bcl-2 homology 3 (BH3) domain; Assays using Bim BH3 peptide resulted in the highest readings (indicating the most binding)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HRK BCL2L1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| BCL2L1 HRK | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| HRK BCL2L1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| BCL2L1 HRK | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| BCL2L1 HRK | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| HRK BCL2L1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID