BAIT
HNRNPK
Hnrpk, KBBP, NOVA
heterogeneous nuclear ribonucleoprotein K
GO Process (8)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of mRNA splicing, via spliceosome [IMP]
- positive regulation of low-density lipoprotein particle receptor biosynthetic process [ISO]
- positive regulation of receptor-mediated endocytosis [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of low-density lipoprotein particle clearance [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
DDX3X
D1Pas1-rs2, Ddx3, Fin14, RP23-158L19.4
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
GO Process (27)
GO Function (14)
GO Component (6)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- DNA duplex unwinding [ISO]
- RNA secondary structure unwinding [ISO]
- cellular response to arsenic-containing substance [ISO]
- cellular response to osmotic stress [ISO]
- chromosome segregation [ISO]
- extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- innate immune response [ISO]
- intracellular signal transduction [ISO]
- intrinsic apoptotic signaling pathway [ISO]
- mature ribosome assembly [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of intrinsic apoptotic signaling pathway [ISO]
- negative regulation of protein complex assembly [ISO]
- negative regulation of translation [ISO]
- positive regulation of G1/S transition of mitotic cell cycle [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of cell growth [ISO]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of translation [ISO]
- positive regulation of translational initiation [ISO]
- positive regulation of viral genome replication [ISO]
- response to virus [ISO]
- stress granule assembly [ISO]
Gene Ontology Molecular Function- ATP-dependent DNA helicase activity [ISO]
- ATP-dependent RNA helicase activity [ISO]
- ATPase activity [ISO]
- DNA binding [ISO]
- RNA binding [ISO]
- RNA stem-loop binding [ISO]
- eukaryotic initiation factor 4E binding [ISO]
- mRNA 5'-UTR binding [ISO]
- poly(A) RNA binding [ISO]
- poly(A) binding [ISO]
- protein binding [IPI]
- ribosomal small subunit binding [ISO]
- transcription factor binding [ISO]
- translation initiation factor binding [ISO]
- ATP-dependent DNA helicase activity [ISO]
- ATP-dependent RNA helicase activity [ISO]
- ATPase activity [ISO]
- DNA binding [ISO]
- RNA binding [ISO]
- RNA stem-loop binding [ISO]
- eukaryotic initiation factor 4E binding [ISO]
- mRNA 5'-UTR binding [ISO]
- poly(A) RNA binding [ISO]
- poly(A) binding [ISO]
- protein binding [IPI]
- ribosomal small subunit binding [ISO]
- transcription factor binding [ISO]
- translation initiation factor binding [ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Beads-free protein immunoprecipitation for a mass spectrometry-based interactome and posttranslational modifications analysis.
Protein immunoprecipitation (IP) coupled with MS provides means to interrogate protein complexes and their posttranslational modifications (PTMs). In a typical protein IP assay antibodies are conjugated to protein A/G beads requiring large amounts of antibodies, tube transfers and centrifugations.As an alternative, we present Matrix-IP, beads-free microplate-based platform with surface-immobilized antibodies. Assay utilizes standard 96-well polypropylene PCR plates that are laboratory-fabricated ... [more]
Proteome Sci Sep. 04, 2015; 13();23 [Pubmed: 26336360]
Throughput
- High Throughput
Curated By
- BioGRID