BAIT
HNRNPK
Hnrpk, KBBP, NOVA
heterogeneous nuclear ribonucleoprotein K
GO Process (8)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of mRNA splicing, via spliceosome [IMP]
- positive regulation of low-density lipoprotein particle receptor biosynthetic process [ISO]
- positive regulation of receptor-mediated endocytosis [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of low-density lipoprotein particle clearance [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
RPS6
S6R
ribosomal protein S6
GO Process (16)
GO Function (4)
GO Component (13)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- T cell differentiation in thymus [IGI, IMP]
- T cell proliferation involved in immune response [IGI, IMP]
- TOR signaling [ISO]
- activation-induced cell death of T cells [IMP]
- erythrocyte development [IMP]
- gastrulation [IGI, IMP]
- glucose homeostasis [IDA]
- mitotic cell cycle checkpoint [IMP]
- mitotic nuclear division [IMP]
- negative regulation of apoptotic process [IMP]
- oogenesis stage [IMP]
- placenta development [IMP]
- positive regulation of apoptotic process [ISO]
- rRNA processing [IMP, ISO]
- ribosomal small subunit biogenesis [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell body [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytosolic small ribosomal subunit [IBA, IDA, ISO]
- dendrite [IDA, ISO]
- membrane [ISO]
- nucleolus [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [IDA]
- polysome [IDA]
- ribonucleoprotein complex [ISO]
- ribosome [IDA]
- small ribosomal subunit [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Beads-free protein immunoprecipitation for a mass spectrometry-based interactome and posttranslational modifications analysis.
Protein immunoprecipitation (IP) coupled with MS provides means to interrogate protein complexes and their posttranslational modifications (PTMs). In a typical protein IP assay antibodies are conjugated to protein A/G beads requiring large amounts of antibodies, tube transfers and centrifugations.As an alternative, we present Matrix-IP, beads-free microplate-based platform with surface-immobilized antibodies. Assay utilizes standard 96-well polypropylene PCR plates that are laboratory-fabricated ... [more]
Proteome Sci Sep. 04, 2015; 13();23 [Pubmed: 26336360]
Throughput
- High Throughput
Curated By
- BioGRID