BAIT
HNRNPK
Hnrpk, KBBP, NOVA
heterogeneous nuclear ribonucleoprotein K
GO Process (8)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of mRNA splicing, via spliceosome [IMP]
- positive regulation of low-density lipoprotein particle receptor biosynthetic process [ISO]
- positive regulation of receptor-mediated endocytosis [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of low-density lipoprotein particle clearance [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
TRIM28
AA408787, KAP-1, KRIP-1, MommeD9, Tif1b, Tif1beta
tripartite motif-containing 28
GO Process (21)
GO Function (13)
GO Component (6)
Gene Ontology Biological Process
- DNA methylation involved in embryo development [IMP]
- DNA repair [ISO]
- convergent extension involved in axis elongation [IMP]
- embryo implantation [IMP]
- embryonic placenta morphogenesis [IMP]
- epithelial to mesenchymal transition [IDA]
- in utero embryonic development [IMP]
- innate immune response [ISO]
- negative regulation of DNA demethylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI]
- negative regulation of transcription, DNA-templated [IDA, IMP, ISO]
- negative regulation of viral release from host cell [ISO]
- positive regulation of DNA repair [ISO]
- positive regulation of transcription factor import into nucleus [ISO]
- positive regulation of transcription, DNA-templated [IDA]
- protein autophosphorylation [IDA]
- protein oligomerization [ISO]
- protein phosphorylation [IDA]
- protein sumoylation [ISO]
- protein ubiquitination [ISO]
- regulation of genetic imprinting [IMP]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- Krueppel-associated box domain binding [ISO]
- chromo shadow domain binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein kinase activity [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- zinc ion binding [ISO]
- DNA binding [IDA, ISO]
- Krueppel-associated box domain binding [ISO]
- chromo shadow domain binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein kinase activity [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Beads-free protein immunoprecipitation for a mass spectrometry-based interactome and posttranslational modifications analysis.
Protein immunoprecipitation (IP) coupled with MS provides means to interrogate protein complexes and their posttranslational modifications (PTMs). In a typical protein IP assay antibodies are conjugated to protein A/G beads requiring large amounts of antibodies, tube transfers and centrifugations.As an alternative, we present Matrix-IP, beads-free microplate-based platform with surface-immobilized antibodies. Assay utilizes standard 96-well polypropylene PCR plates that are laboratory-fabricated ... [more]
Proteome Sci Sep. 04, 2015; 13();23 [Pubmed: 26336360]
Throughput
- High Throughput
Curated By
- BioGRID