BAIT
HNRNPK
Hnrpk, KBBP, NOVA
heterogeneous nuclear ribonucleoprotein K
GO Process (8)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of mRNA splicing, via spliceosome [IMP]
- positive regulation of low-density lipoprotein particle receptor biosynthetic process [ISO]
- positive regulation of receptor-mediated endocytosis [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of low-density lipoprotein particle clearance [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
PRPF19
AA617263, AL024362, D19Wsu55e, NMP200, PSO4, Prp19, Snev
PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
GO Process (17)
GO Function (5)
GO Component (12)
Gene Ontology Biological Process
- cellular protein localization [ISO]
- double-strand break repair via nonhomologous end joining [ISO]
- generation of catalytic spliceosome for first transesterification step [IBA]
- inner cell mass cell proliferation [IMP]
- lipid biosynthetic process [IDA]
- mRNA splicing, via spliceosome [ISO]
- negative regulation of neuron differentiation [IDA]
- positive regulation of astrocyte differentiation [IDA]
- positive regulation of mRNA splicing, via spliceosome [IMP]
- positive regulation of neuron differentiation [ISA]
- proteasomal protein catabolic process [IMP, ISO]
- protein K63-linked ubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IBA]
- signal transduction involved in DNA damage checkpoint [ISO]
- spliceosomal complex assembly [ISO]
- spliceosomal tri-snRNP complex assembly [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- DNA replication factor A complex [ISO]
- Prp19 complex [IBA, ISO]
- U2-type catalytic step 1 spliceosome [IBA]
- catalytic step 2 spliceosome [IBA, ISO]
- cytoplasm [IDA, ISO]
- lipid particle [IDA]
- membrane [ISO]
- nuclear speck [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISA, ISO]
- site of double-strand break [ISO]
- spliceosomal complex [IDA, ISA]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Beads-free protein immunoprecipitation for a mass spectrometry-based interactome and posttranslational modifications analysis.
Protein immunoprecipitation (IP) coupled with MS provides means to interrogate protein complexes and their posttranslational modifications (PTMs). In a typical protein IP assay antibodies are conjugated to protein A/G beads requiring large amounts of antibodies, tube transfers and centrifugations.As an alternative, we present Matrix-IP, beads-free microplate-based platform with surface-immobilized antibodies. Assay utilizes standard 96-well polypropylene PCR plates that are laboratory-fabricated ... [more]
Proteome Sci Sep. 04, 2015; 13();23 [Pubmed: 26336360]
Throughput
- High Throughput
Curated By
- BioGRID