BAIT
HNRNPK
Hnrpk, KBBP, NOVA
heterogeneous nuclear ribonucleoprotein K
GO Process (8)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of mRNA splicing, via spliceosome [IMP]
- positive regulation of low-density lipoprotein particle receptor biosynthetic process [ISO]
- positive regulation of receptor-mediated endocytosis [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of low-density lipoprotein particle clearance [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- single-stranded DNA binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
RPA1
5031405K23Rik, 70kDa, AA589576, AW557552, RF-A, RP-A, Rpa, RP23-113D4.1
replication protein A1
GO Process (12)
GO Function (3)
GO Component (8)
Gene Ontology Biological Process
- DNA replication [ISO]
- base-excision repair [ISO]
- chromosome organization [IMP]
- double-strand break repair via homologous recombination [IMP, ISO]
- hemopoiesis [IMP]
- homeostasis of number of cells within a tissue [IMP]
- in utero embryonic development [IMP]
- meiotic nuclear division [IDA]
- mismatch repair [ISO]
- nucleotide-excision repair [ISO]
- positive regulation of cell proliferation [IMP]
- telomere maintenance [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Beads-free protein immunoprecipitation for a mass spectrometry-based interactome and posttranslational modifications analysis.
Protein immunoprecipitation (IP) coupled with MS provides means to interrogate protein complexes and their posttranslational modifications (PTMs). In a typical protein IP assay antibodies are conjugated to protein A/G beads requiring large amounts of antibodies, tube transfers and centrifugations.As an alternative, we present Matrix-IP, beads-free microplate-based platform with surface-immobilized antibodies. Assay utilizes standard 96-well polypropylene PCR plates that are laboratory-fabricated ... [more]
Proteome Sci Sep. 04, 2015; 13();23 [Pubmed: 26336360]
Throughput
- High Throughput
Curated By
- BioGRID