BAIT
GJA1
Cx43
gap junction protein, alpha 1
GO Process (61)
GO Function (10)
GO Component (28)
Gene Ontology Biological Process
- ATP transport [IMP]
- adult heart development [ISO]
- apoptotic process [IMP]
- atrial ventricular junction remodeling [ISO]
- blood vessel morphogenesis [ISO]
- cardiac conduction [ISO]
- cell communication [IMP]
- cell communication by chemical coupling [ISO]
- cell communication by electrical coupling [ISO]
- cell-cell junction organization [ISO]
- cell-cell signaling [IDA, ISO]
- cellular response to mechanical stimulus [IEP]
- chronic inflammatory response [IMP]
- embryonic digit morphogenesis [ISO]
- embryonic heart tube development [ISO]
- endothelium development [IEP]
- epithelial cell maturation [ISO]
- gap junction assembly [TAS]
- heart development [IEP, ISO]
- heart looping [ISO]
- in utero embryonic development [ISO]
- lens development in camera-type eye [ISO]
- milk ejection [ISO]
- negative regulation of DNA biosynthetic process [IDA]
- negative regulation of cardiac muscle cell proliferation [IMP]
- negative regulation of cell proliferation [IDA]
- negative regulation of endothelial cell proliferation [IMP]
- negative regulation of gene expression [ISO]
- negative regulation of wound healing [IMP]
- neuron migration [ISO]
- neuron projection morphogenesis [IMP]
- osteoblast differentiation [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of behavioral fear response [IMP]
- positive regulation of cell communication by chemical coupling [IMP]
- positive regulation of cytosolic calcium ion concentration [IMP]
- positive regulation of gene expression [ISO]
- positive regulation of glomerular filtration [IMP]
- positive regulation of insulin secretion [IMP]
- positive regulation of osteoblast differentiation [ISO]
- positive regulation of protein catabolic process [IDA]
- positive regulation of striated muscle tissue development [ISO]
- positive regulation of vasoconstriction [IMP]
- positive regulation of vasodilation [IMP]
- protein oligomerization [IDA]
- regulation of atrial cardiac muscle cell membrane depolarization [ISO]
- regulation of bone mineralization [ISO]
- regulation of bone remodeling [ISO]
- regulation of calcium ion transport [IMP]
- regulation of heart contraction [ISO]
- regulation of tight junction assembly [IMP]
- regulation of ventricular cardiac muscle cell membrane depolarization [ISO]
- regulation of ventricular cardiac muscle cell membrane repolarization [ISO]
- response to fluid shear stress [IEP]
- response to glucose [IEP]
- response to pH [IDA]
- response to peptide hormone [IEP]
- signal transduction [ISO]
- skeletal muscle tissue regeneration [ISO]
- transmembrane transport [IDA, ISO]
- vascular transport [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA, ISO]
- Golgi membrane [TAS]
- Golgi-associated vesicle membrane [TAS]
- apical plasma membrane [ISO]
- cell junction [ISO]
- cell-cell junction [ISO]
- connexon complex [IDA]
- contractile fiber [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- early endosome [IDA]
- endoplasmic reticulum membrane [TAS]
- endosome [IDA]
- extracellular vesicular exosome [ISO]
- fascia adherens [IDA, ISO]
- focal adhesion [ISO]
- gap junction [IDA, ISO]
- integral component of plasma membrane [TAS]
- intercalated disc [ISO]
- intermediate filament [ISO]
- late endosome [IDA]
- lateral plasma membrane [ISO]
- lysosome [IDA]
- membrane [IDA, ISO]
- membrane raft [IDA]
- mitochondrial outer membrane [IDA]
- multivesicular body [IDA]
- plasma membrane [IDA, IMP, ISO, TAS]
Rattus norvegicus
PREY
HSPA8
Hsc70
heat shock 70kDa protein 8
GO Process (10)
GO Function (13)
GO Component (18)
Gene Ontology Biological Process
- ATP catabolic process [IDA, ISO]
- chaperone mediated protein folding requiring cofactor [IDA, ISO]
- chaperone-mediated protein folding [IDA]
- clathrin coat disassembly [ISO]
- negative regulation of fibril organization [ISO]
- negative regulation of transcription, DNA-templated [ISO, ISS]
- positive regulation of mRNA splicing, via spliceosome [ISO]
- protein folding [ISO]
- protein refolding [ISO]
- regulation of cell cycle [ISO]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA, ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IMP, ISO]
- ADP binding [IDA]
- ATP binding [IDA, ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IMP, ISO]
Gene Ontology Cellular Component
- Prp19 complex [ISO, ISS]
- blood microparticle [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [IDA, ISO]
- focal adhesion [ISO]
- intracellular [ISO]
- membrane [ISO]
- myelin sheath [ISO]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleus [ISO, ISS]
- protein complex [IDA]
- ribonucleoprotein complex [ISO, ISS]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
- ubiquitin ligase complex [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart.
The coordinated and synchronized cardiac muscle contraction relies on an efficient gap junction-mediated intercellular communication (GJIC) between cardiomyocytes, which involves the rapid anisotropic impulse propagation through connexin (Cx)-containing channels, namely of Cx43, the most abundant Cx in the heart. Expectedly, disturbing mechanisms that affect channel activity, localization and turnover of Cx43 have been implicated in several cardiomyopathies, such as myocardial ... [more]
Mol. Cell Proteomics Nov. 01, 2015; 14(11);3040-55 [Pubmed: 26316108]
Throughput
- High Throughput
Additional Notes
- Identification of Cx43-binding partners using the SWATH-MS method.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GJA1 HSPA8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID