BAIT

GJA1

Cx43
gap junction protein, alpha 1
GO Process (61)
GO Function (10)
GO Component (28)

Gene Ontology Biological Process

Rattus norvegicus
PREY

MCCC2

methylcrotonoyl-CoA carboxylase 2 (beta)
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart.

Martins-Marques T, Anjo SI, Pereira P, Manadas B, Girao H

The coordinated and synchronized cardiac muscle contraction relies on an efficient gap junction-mediated intercellular communication (GJIC) between cardiomyocytes, which involves the rapid anisotropic impulse propagation through connexin (Cx)-containing channels, namely of Cx43, the most abundant Cx in the heart. Expectedly, disturbing mechanisms that affect channel activity, localization and turnover of Cx43 have been implicated in several cardiomyopathies, such as myocardial ... [more]

Mol. Cell Proteomics Nov. 01, 2015; 14(11);3040-55 [Pubmed: 26316108]

Throughput

  • High Throughput

Additional Notes

  • Identification of Cx43-binding partners using the SWATH-MS method.

Curated By

  • BioGRID