BAIT
GJA1
Cx43
gap junction protein, alpha 1
GO Process (61)
GO Function (10)
GO Component (28)
Gene Ontology Biological Process
- ATP transport [IMP]
- adult heart development [ISO]
- apoptotic process [IMP]
- atrial ventricular junction remodeling [ISO]
- blood vessel morphogenesis [ISO]
- cardiac conduction [ISO]
- cell communication [IMP]
- cell communication by chemical coupling [ISO]
- cell communication by electrical coupling [ISO]
- cell-cell junction organization [ISO]
- cell-cell signaling [IDA, ISO]
- cellular response to mechanical stimulus [IEP]
- chronic inflammatory response [IMP]
- embryonic digit morphogenesis [ISO]
- embryonic heart tube development [ISO]
- endothelium development [IEP]
- epithelial cell maturation [ISO]
- gap junction assembly [TAS]
- heart development [IEP, ISO]
- heart looping [ISO]
- in utero embryonic development [ISO]
- lens development in camera-type eye [ISO]
- milk ejection [ISO]
- negative regulation of DNA biosynthetic process [IDA]
- negative regulation of cardiac muscle cell proliferation [IMP]
- negative regulation of cell proliferation [IDA]
- negative regulation of endothelial cell proliferation [IMP]
- negative regulation of gene expression [ISO]
- negative regulation of wound healing [IMP]
- neuron migration [ISO]
- neuron projection morphogenesis [IMP]
- osteoblast differentiation [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of behavioral fear response [IMP]
- positive regulation of cell communication by chemical coupling [IMP]
- positive regulation of cytosolic calcium ion concentration [IMP]
- positive regulation of gene expression [ISO]
- positive regulation of glomerular filtration [IMP]
- positive regulation of insulin secretion [IMP]
- positive regulation of osteoblast differentiation [ISO]
- positive regulation of protein catabolic process [IDA]
- positive regulation of striated muscle tissue development [ISO]
- positive regulation of vasoconstriction [IMP]
- positive regulation of vasodilation [IMP]
- protein oligomerization [IDA]
- regulation of atrial cardiac muscle cell membrane depolarization [ISO]
- regulation of bone mineralization [ISO]
- regulation of bone remodeling [ISO]
- regulation of calcium ion transport [IMP]
- regulation of heart contraction [ISO]
- regulation of tight junction assembly [IMP]
- regulation of ventricular cardiac muscle cell membrane depolarization [ISO]
- regulation of ventricular cardiac muscle cell membrane repolarization [ISO]
- response to fluid shear stress [IEP]
- response to glucose [IEP]
- response to pH [IDA]
- response to peptide hormone [IEP]
- signal transduction [ISO]
- skeletal muscle tissue regeneration [ISO]
- transmembrane transport [IDA, ISO]
- vascular transport [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA, ISO]
- Golgi membrane [TAS]
- Golgi-associated vesicle membrane [TAS]
- apical plasma membrane [ISO]
- cell junction [ISO]
- cell-cell junction [ISO]
- connexon complex [IDA]
- contractile fiber [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- early endosome [IDA]
- endoplasmic reticulum membrane [TAS]
- endosome [IDA]
- extracellular vesicular exosome [ISO]
- fascia adherens [IDA, ISO]
- focal adhesion [ISO]
- gap junction [IDA, ISO]
- integral component of plasma membrane [TAS]
- intercalated disc [ISO]
- intermediate filament [ISO]
- late endosome [IDA]
- lateral plasma membrane [ISO]
- lysosome [IDA]
- membrane [IDA, ISO]
- membrane raft [IDA]
- mitochondrial outer membrane [IDA]
- multivesicular body [IDA]
- plasma membrane [IDA, IMP, ISO, TAS]
Rattus norvegicus
PREY
RYR2
RYR-2, RyR
ryanodine receptor 2, cardiac
GO Process (42)
GO Function (16)
GO Component (20)
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- Purkinje myocyte to ventricular cardiac muscle cell signaling [ISO]
- calcium ion transmembrane transport [IMP, ISO]
- calcium ion transport [ISO, ISS]
- calcium ion transport into cytosol [ISO]
- calcium-mediated signaling [ISO, ISS]
- calcium-mediated signaling using intracellular calcium source [ISO]
- canonical Wnt signaling pathway [ISO]
- cardiac muscle contraction [ISO]
- cardiac muscle hypertrophy [ISO]
- cellular calcium ion homeostasis [ISO, ISS]
- cellular response to caffeine [ISO, ISS]
- cytosolic calcium ion homeostasis [IMP]
- cytosolic calcium ion transport [ISO]
- detection of calcium ion [ISO]
- embryonic heart tube morphogenesis [ISO, ISS]
- establishment of protein localization to endoplasmic reticulum [ISO]
- ion transmembrane transport [IMP]
- left ventricular cardiac muscle tissue morphogenesis [ISO]
- manganese ion transmembrane transport [IMP]
- negative regulation of cytosolic calcium ion concentration [IDA]
- positive regulation of calcium-transporting ATPase activity [ISO]
- positive regulation of heart rate [ISO]
- positive regulation of ryanodine-sensitive calcium-release channel activity by adrenergic receptor signaling pathway involved in positive regulation of cardiac muscle contraction [ISO]
- positive regulation of sequestering of calcium ion [ISO]
- positive regulation of the force of heart contraction [ISO]
- regulation of cardiac muscle contraction [ISO]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [ISO]
- regulation of heart rate [ISO]
- release of sequestered calcium ion into cytosol [ISO]
- release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISO]
- response to caffeine [ISO]
- response to calcium ion [IDA]
- response to drug [IEP]
- response to hypoxia [ISO]
- response to magnesium ion [IDA]
- response to muscle stretch [ISO]
- response to nutrient [IEP]
- response to redox state [ISO]
- sarcoplasmic reticulum calcium ion transport [IMP]
- type B pancreatic cell apoptotic process [ISO]
- ventricular cardiac muscle cell action potential [ISO]
Gene Ontology Molecular Function- calcium channel activity [ISO, ISS]
- calcium-induced calcium release activity [IDA, ISO]
- calcium-release channel activity [ISO]
- calmodulin binding [ISO]
- enzyme binding [ISO]
- identical protein binding [ISO]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- organic cyclic compound binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase binding [ISO]
- protein self-association [ISO]
- ryanodine-sensitive calcium-release channel activity [IDA, IMP, ISO]
- scaffold protein binding [IPI]
- suramin binding [ISO]
- calcium channel activity [ISO, ISS]
- calcium-induced calcium release activity [IDA, ISO]
- calcium-release channel activity [ISO]
- calmodulin binding [ISO]
- enzyme binding [ISO]
- identical protein binding [ISO]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- organic cyclic compound binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase binding [ISO]
- protein self-association [ISO]
- ryanodine-sensitive calcium-release channel activity [IDA, IMP, ISO]
- scaffold protein binding [IPI]
- suramin binding [ISO]
Gene Ontology Cellular Component
- A band [IDA]
- Z disc [IDA, ISO]
- calcium channel complex [ISO]
- cell [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- endoplasmic reticulum [IDA]
- extracellular vesicular exosome [ISO]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- intracellular [ISO]
- membrane [ISO]
- neuron projection [IDA]
- nuclear envelope [IDA]
- plasma membrane [IDA]
- protein complex [IDA, ISO]
- sarcolemma [IDA]
- sarcomere [ISO]
- sarcoplasmic reticulum [IDA, ISO]
- sarcoplasmic reticulum membrane [IDA, ISO]
- smooth endoplasmic reticulum [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart.
The coordinated and synchronized cardiac muscle contraction relies on an efficient gap junction-mediated intercellular communication (GJIC) between cardiomyocytes, which involves the rapid anisotropic impulse propagation through connexin (Cx)-containing channels, namely of Cx43, the most abundant Cx in the heart. Expectedly, disturbing mechanisms that affect channel activity, localization and turnover of Cx43 have been implicated in several cardiomyopathies, such as myocardial ... [more]
Mol. Cell Proteomics Nov. 01, 2015; 14(11);3040-55 [Pubmed: 26316108]
Throughput
- High Throughput
Additional Notes
- Identification of Cx43-binding partners using the SWATH-MS method.
Curated By
- BioGRID