A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.

Hultquist JF, Schumann K, Woo JM, Manganaro L, McGregor MJ, Doudna J, Simon V, Krogan NJ, Marson A

New genetic tools are needed to understand the functional interactions between HIV and human host factors in primary cells. We recently developed a method to edit the genome of primary CD4+ TÂ cells by electroporation of CRISPR/Cas9 ribonucleoproteins (RNPs). Here, we adapted this methodology to a high-throughput platform for the efficient, arrayed editing of candidate host factors. CXCR4 or CCR5 knockout ... [more]

Cell Rep Dec. 25, 2015; 17(5);1438-1452 [Pubmed: 27783955]

Throughput

Low Throughput

Ontology Terms

biological process: response to virus (GO:0009615)

Additional Notes

double mutants show dual viral resistance combining restriction of the CXCR4-tropic virus as effectively as the CXCR4 knock-out cells and restriction of the VSV-G pseudotyped virus as effectively as the LEDGF knockout cells

Curated By

BioGRID

Interaction Summary

CXCR4

Gene Ontology Biological Process

Gene Ontology Molecular Function

C-X-C chemokine receptor activity [NAS]G-protein coupled receptor activity [TAS]actin binding [IDA]coreceptor activity [TAS]myosin light chain binding [IDA]protein binding [IPI]ubiquitin binding [IDA]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

PSIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription coactivator activity [IDA]activating transcription factor binding [IDA]chromatin binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]supercoiled DNA binding [ISS]