BAIT

HSPA1A

HEL-S-103, HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, DAQB-147D11.1
heat shock 70kDa protein 1A
GO Process (20)
GO Function (13)
GO Component (17)
Homo sapiens
PREY

BAG1

BAG-1, HAP, RAP46, RP11-326F20.2
BCL2-associated athanogene
GO Process (4)
GO Function (2)
GO Component (1)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The cochaperone Bag-1L enhances androgen receptor action via interaction with the NH2-terminal region of the receptor.

Shatkina L, Mink S, Rogatsch H, Klocker H, Langer G, Nestl A, Cato AC

Members of the Bag-1 family of cochaperones regulate diverse cellular processes including the action of steroid hormone receptors. The largest member of this family, Bag-1L, enhances the transactivation function of the androgen receptor. This occurs primarily through interaction with the NH(2) and COOH termini of the receptor. At the NH(2) terminus of the receptor, Bag-1L interacts with a region termed ... [more]

Mol. Cell. Biol. Oct. 01, 2003; 23(20);7189-97 [Pubmed: 14517289]

Throughput

  • Low Throughput

Additional Notes

  • Surface plasmon resonance and transfection studies showed that the molecular chaperone Hsp70 contributes to the binding of Bag-1L to tau 5 and to the regulation of the transactivation function of the androgen receptor. Chromatin immunoprecipitation studie

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BAG1 HSPA1A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High3.25BioGRID
3379798

Curated By

  • BioGRID