MLEC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DDOST
Gene Ontology Biological Process
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- T cell activation [IDA]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation [IDA]
- protein N-linked glycosylation via asparagine [IC, TAS]
- protein glycosylation [ISS]
- response to cytokine [IDA]
- translation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Malectin forms a complex with ribophorin I for enhanced association with misfolded glycoproteins.
Malectin is an endoplasmic reticulum-resident lectin, which recognizes di-glucosylated Glc(2)Man(9)GlcNAc(2) (G2M9) N-glycans on newly synthesized glycoproteins. We previously demonstrated that malectin preferentially associates with misfolded glycoproteins and inhibits their secretion (Chen, Y., Hu, D., Yabe, R., Tateno, H., Qin, S. Y., Matsumoto, N., Hirabayashi, J., and Yamamoto, K. (2011) Mol. Biol. Cell 22, 3559-3570). The sugar binding activity of malectin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DDOST MLEC | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3353691 |
Curated By
- BioGRID