BAIT
SFN
YWHAS
stratifin
GO Process (13)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- apoptotic process [TAS]
- establishment of skin barrier [ISS]
- intrinsic apoptotic signaling pathway [TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- membrane organization [TAS]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- negative regulation of protein kinase activity [TAS]
- negative regulation of protein serine/threonine kinase activity [TAS]
- positive regulation of epidermal cell differentiation [ISS]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- regulation of epidermal cell division [ISS]
- release of cytochrome c from mitochondria [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
IRS1
HIRS-1
insulin receptor substrate 1
GO Process (25)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- cellular response to insulin stimulus [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- glucose homeostasis [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IDA, IMP, IPI, TAS]
- insulin-like growth factor receptor signaling pathway [IPI]
- negative regulation of insulin receptor signaling pathway [ISS]
- negative regulation of insulin secretion [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol 3-kinase signaling [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of cell proliferation [NAS]
- positive regulation of fatty acid beta-oxidation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose import in response to insulin stimulus [IDA]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP, NAS]
- positive regulation of insulin receptor signaling pathway [ISS]
- positive regulation of phosphatidylinositol 3-kinase activity [ISS]
- response to insulin [IDA]
- response to peptide hormone [ISS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer.
To comprehensively identify proteins interacting with 14-3-3 sigma in vivo, tandem affinity purification and the multidimensional protein identification technology were combined to characterize 117 proteins associated with 14-3-3 sigma in human cells. The majority of identified proteins contained one or several phosphorylatable 14-3-3-binding sites indicating a potential direct interaction with 14-3-3 sigma. 25 proteins were not previously assigned to any ... [more]
Mol. Cell Proteomics Jun. 01, 2005; 4(6);785-95 [Pubmed: 15778465]
Throughput
- Low Throughput
Additional Notes
- Tandem affinity purification (TAP) and the multidimensional protein identification technology (MudPIT)
Curated By
- BioGRID