BAIT
CRBN
MRT2, MRT2A, AD-006
cereblon
GO Process (2)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Lenalidomide causes selective degradation of IKZF1 and IKZF3 in multiple myeloma cells.
Lenalidomide is a drug with clinical efficacy in multiple myeloma and other B cell neoplasms, but its mechanism of action is unknown. Using quantitative proteomics, we found that lenalidomide causes selective ubiquitination and degradation of two lymphoid transcription factors, IKZF1 and IKZF3, by the CRBN-CRL4 ubiquitin ligase. IKZF1 and IKZF3 are essential transcription factors in multiple myeloma. A single amino ... [more]
Science Jan. 17, 2014; 343(6168);301-5 [Pubmed: 24292625]
Throughput
- Low Throughput
Curated By
- BioGRID