Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer.

Benzinger A, Muster N, Koch HB, Yates JR, Hermeking H

To comprehensively identify proteins interacting with 14-3-3 sigma in vivo, tandem affinity purification and the multidimensional protein identification technology were combined to characterize 117 proteins associated with 14-3-3 sigma in human cells. The majority of identified proteins contained one or several phosphorylatable 14-3-3-binding sites indicating a potential direct interaction with 14-3-3 sigma. 25 proteins were not previously assigned to any ... [more]

Mol. Cell Proteomics Jun. 01, 2005; 4(6);785-95 [Pubmed: 15778465]

Throughput

  • Low Throughput

Additional Notes

  • Tandem affinity purification (TAP) and the multidimensional protein identification technology (MudPIT)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LAD1 SFN
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.3664BioGRID
3220372

Curated By

  • BioGRID