Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

RAD51 localization and activation following DNA damage.

Tarsounas M, Davies AA, West SC

The efficient repair of double-strand breaks in DNA is critical for the maintenance of genome stability. In response to ionizing radiation and other DNA-damaging agents, the RAD51 protein, which is essential for homologous recombination, relocalizes within the nucleus to form distinct foci that can be visualized by microscopy and are thought to represent sites where repair reactions take place. The ... [more]

Philos. Trans. R. Soc. Lond., B, Biol. Sci. Jan. 29, 2004; 359(1441);87-93 [Pubmed: 15065660]

Throughput

  • Low Throughput

Additional Notes

  • The formation of RAD51 foci in response to DNA damage is dependent upon BRCA2 and a series of proteins known as the RAD51 paralogues (RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3)

Curated By

  • BioGRID