BAIT

HIST2H3A

H3/n, H3/o
histone cluster 2, H3a
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

NAP1L4

NAP1L4b, NAP2, NAP2L, hNAP2
nucleosome assembly protein 1-like 4
GO Process (0)
GO Function (3)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Chemical Proteomics Approach to Reveal Direct Protein-Protein Interactions in Living Cells.

Kleiner RE, Hang LE, Molloy KR, Chait BT, Kapoor TM

Protein-protein interactions mediate essential cellular processes, however the detection of native interactions is challenging since they are often low affinity and context dependent. Here, we develop a chemical proteomics approach in vivo CLASPI [iCLASPI] (in vivo crosslinking-assisted and stable isotope labeling by amino acids in cell culture [SILAC]-based protein identification) relying upon photo-crosslinking, amber suppression, and SILAC-based quantitative proteomics to profile context-dependent ... [more]

Cell Chem Biol Jan. 18, 2018; 25(1);110-120.e3 [Pubmed: 29104064]

Throughput

  • High Throughput

Additional Notes

  • Source of HIST2H3A not clear

Curated By

  • BioGRID