BAIT

HPR1

TRF1, L000000808, YDR138W
Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
Saccharomyces cerevisiae (S288c)
PREY

HHF1

histone H4, L000000770, YBR009C
Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Histone Mutants Separate R Loop Formation from Genome Instability Induction.

Garcia-Pichardo D, Canas JC, Garcia-Rubio ML, Gomez-Gonzalez B, Rondon AG, Aguilera A

R loops have positive physiological roles, but they can also be deleterious by causing genome instability, and the mechanisms for this are unknown. Here we identified yeast histone H3 and H4 mutations that facilitate R loops but do not cause instability. R loops containing single-stranded DNA (ssDNA), versus RNA-DNA hybrids alone, were demonstrated using ssDNA-specific human AID and bisulfite. Notably, they ... [more]

Mol. Cell Jun. 01, 2017; 66(5);597-609.e5 [Pubmed: 28575656]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chromosome/plasmid maintenance (APO:0000143)

Additional Notes

  • hyper-recombination phenotypes of R loop-accumulating hpr1 and sen1-1 mutants were suppressed by the H3K9-23A, H3delta1-28, or H4K31Q mutations

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HPR1 HHF1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
160823

Curated By

  • BioGRID