PREY

ATM

AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1
ATM serine/threonine kinase
Homo sapiens

Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Substrate specificities and identification of putative substrates of ATM kinase family members.

Kim ST, Lim DS, Canman CE, Kastan MB

Ataxia telangiectasia mutated (ATM) phosphorylates p53 protein in response to ionizing radiation, but the complex phenotype of AT cells suggests that it must have other cellular substrates as well. To identify substrates for ATM and the related kinases ATR and DNA-PK, we optimized in vitro kinase assays and developed a rapid peptide screening method to determine general phosphorylation consensus sequences. ... [more]

J. Biol. Chem. Dec. 31, 1999; 274(53);37538-43 [Pubmed: 10608806]

Throughput

  • Low Throughput

Additional Notes

  • in vitro kinase assay using GST-peptide substrates

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ATR ATM
Biochemical Activity
Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Low-BioGRID
467651
ATM ATR
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
-

Curated By

  • BioGRID