BAIT

BANF1

BAF, BCRP1, D14S1460, NGPS
barrier to autointegration factor 1
Homo sapiens
PREY

HIST1H1C

H1.2, H1C, H1F2, H1s-1
histone cluster 1, H1c
GO Process (0)
GO Function (3)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Binding of barrier to autointegration factor (BAF) to histone H3 and selected linker histones including H1.1.

Montes de Oca R, Lee KK, Wilson KL

Barrier to autointegration factor (BAF) is an essential conserved double-stranded DNA-binding protein in metazoans. BAF binds directly to LEM domain nuclear proteins (e.g. LAP2, Emerin, and MAN1), lamin A, homeodomain transcription factors, and human immunodeficiency virus type 1-encoded proteins. BAF influences higher order chromatin structure and is required to assemble nuclei. BAF also facilitates retroviral preintegration complex insertion into target ... [more]

J. Biol. Chem. Dec. 23, 2005; 280(51);42252-62 [Pubmed: 16203725]

Throughput

  • Low Throughput

Curated By

  • BioGRID