BAIT

VPS30

APG6, ATG6, VPT30, L000003545, YPL120W
Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has a novel globular fold that is essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of the higher eukaryotic gene Beclin 1
Saccharomyces cerevisiae (S288c)
PREY

PIB2

S000007475, YGL023C
Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Pib2 and EGO Complex are both required for activation of TORC1.

Varlakhanova NV, Mihalevic M, Bernstein KA, Ford MGJ

The TORC1 complex is a key regulator of cell growth and metabolism in Saccharomyces cerevisiae The vacuole-associated EGO Complex couples activation of TORC1 to the availability of amino acids, specifically glutamine and leucine. EGO Complex is also essential for reactivation of TORC1 following rapamycin-induced growth arrest and for its distribution on the vacuolar membrane. Pib2, a FYVE-containing PI3P-binding protein, is ... [more]

J. Cell. Sci. Oct. 09, 2017; (); [Pubmed: 28993463]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • pib2 was lethal when overexpressed in the mutant background

Curated By

  • BioGRID