Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Human ALKBH4 interacts with proteins associated with transcription.

Bjornstad LG, Meza TJ, Otterlei M, Olafsrud SM, Meza-Zepeda LA, Falnes P O

The Fe(II)- and 2-oxoglutarate (2OG)-dependent dioxygenase AlkB from E. coli is a demethylase which repairs alkyl lesions in DNA, as well as RNA, through a direct reversal mechanism. Humans possess nine AlkB homologs (ALKBH1-8 and FTO). ALKBH2 and ALKBH3 display demethylase activities corresponding to that of AlkB, and both ALKBH8 and FTO are RNA modification enzymes. The biochemical functions of ... [more]

PLoS ONE Nov. 13, 2012; 7(11);e49045 [Pubmed: 23145062]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ALKBH4

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [IDA]demethylase activity [TAS]oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]

Gene Ontology Cellular Component

EIF3C

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]translation initiation factor activity [IC, IDA]

Gene Ontology Cellular Component

Two-hybrid

Publication

Human ALKBH4 interacts with proteins associated with transcription.

Throughput

Curated By

actin binding [IDA]
demethylase activity [TAS]
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]

poly(A) RNA binding [IDA]
protein binding [IPI]
translation initiation factor activity [IC, IDA]