BAIT

CIN8

KSL2, SDS15, kinesin motor protein CIN8, L000000340, YEL061C
Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation
Saccharomyces cerevisiae (S288c)
PREY

UBA1

E1 ubiquitin-activating protein UBA1, L000002405, YKL210W
Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
UPS Project
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

New ubiquitin-dependent mechanisms regulating the Aurora B-Protein Phosphatase 1 balance in Saccharomyces cerevisiae.

Ravindran R, Polk P, Robinson LC, Tatchell K

Protein ubiquitylation regulates many cellular processes, including cell division. We report here a novel mutation altering the S. cerevisiae E1 ubiquitin activating enzyme (uba1-W928R) that suppresses the temperature sensitivity and chromosome loss phenotype of a well-characterized Aurora B mutant (ip1-2). The uba1-W928R mutation increases Histone H3-S10 phosphorylation in the ipl1-2 strain, indicating that uba1-W928R acts by increasing Ipl1 activity and/or ... [more]

J. Cell. Sci. Jul. 27, 2018; (); [Pubmed: 30054382]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Curated By

  • BioGRID