EXOSC2
Gene Ontology Biological Process
- CUT catabolic process [IBA]
- RNA metabolic process [TAS]
- U4 snRNA 3'-end processing [IBA]
- exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [IBA, TAS]
- exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IBA]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear polyadenylation-dependent rRNA catabolic process [IBA]
- nuclear polyadenylation-dependent tRNA catabolic process [IBA]
- nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription [IBA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [IBA]
- polyadenylation-dependent snoRNA 3'-end processing [IBA]
- positive regulation of cell growth [IMP]
- rRNA processing [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MPHOSPH6
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Mapping the Genetic Landscape of Human Cells.
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]
Quantitative Score
- -4.16981654 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- cell type: jurkat cell (BTO:0000661)
Additional Notes
- CRISPR GI screen
- Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative/positive genetic interaction
- Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
- Jurkat cell line Replicate Average GI score = -4.16981654
- Library: CRISPRi v1
- Significance Threshold: (positive genetic interaction) 3
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EXOSC2 MPHOSPH6 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1450329 | |
| MPHOSPH6 EXOSC2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3092533 | |
| EXOSC2 MPHOSPH6 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 103.131 | BioGRID | 2941749 | |
| EXOSC2 MPHOSPH6 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3450517 | |
| MPHOSPH6 EXOSC2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1 | BioGRID | 1261968 | |
| MPHOSPH6 EXOSC2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID