Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Mapping the Genetic Landscape of Human Cells.
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]
Quantitative Score
- -3.291270214 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- cell type: k-562 cell (BTO:0000664)
Additional Notes
- CRISPR GI screen
- Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative/positive genetic interaction
- Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
- K562 cell line Replicate Average GI score = -3.291270214
- Library: CRISPRi v1
- Significance Threshold: (positive genetic interaction) 3
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FAM50A FAM50A | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 4.6044 | BioGRID | 2457240 |
Curated By
- BioGRID