BAIT
GADD45A
DDIT1, GADD45, RP5-975D15.1
growth arrest and DNA-damage-inducible, alpha
GO Process (11)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- DNA repair [TAS]
- activation of MAPKKK activity [IDA]
- apoptotic process [TAS]
- cellular response to ionizing radiation [IMP]
- cellular response to mechanical stimulus [IEP]
- positive regulation of JNK cascade [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of p38MAPK cascade [IDA]
- positive regulation of reactive oxygen species metabolic process [IMP]
- regulation of cyclin-dependent protein serine/threonine kinase activity [TAS]
- signal transduction in response to DNA damage [IMP]
Gene Ontology Molecular Function
Homo sapiens
PREY
PPARA
4933429D07Rik, AW742785, Nr1c1, PPAR-alpha, PPARalpha, Ppar
peroxisome proliferator activated receptor alpha
GO Process (35)
GO Function (21)
GO Component (1)
Gene Ontology Biological Process
- behavioral response to nicotine [ISO]
- circadian regulation of gene expression [IDA]
- enamel mineralization [IMP]
- epidermis development [IMP]
- fatty acid metabolic process [IMP, ISO, TAS]
- glucose metabolic process [TAS]
- intracellular receptor signaling pathway [ISO]
- lipid metabolic process [TAS]
- lipoprotein metabolic process [ISO]
- negative regulation of appetite [IMP]
- negative regulation of blood pressure [ISO]
- negative regulation of cholesterol storage [ISO]
- negative regulation of macrophage derived foam cell differentiation [ISO]
- negative regulation of neuron death [ISO]
- negative regulation of protein binding [ISO]
- negative regulation of receptor biosynthetic process [ISO]
- negative regulation of sequestering of triglyceride [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription regulatory region DNA binding [ISO]
- positive regulation of fatty acid oxidation [IMP]
- positive regulation of gluconeogenesis [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IMP, ISO]
- regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of circadian rhythm [IDA]
- regulation of fatty acid metabolic process [IMP]
- regulation of gene expression [IMP]
- regulation of glycolytic by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [ISO, TAS]
- response to hypoxia [ISO]
- response to insulin [ISO]
- steroid hormone mediated signaling pathway [ISO]
- transcription from RNA polymerase II promoter [IDA]
- wound healing [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- MDM2/MDM4 family protein binding [ISO]
- NFAT protein binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- drug binding [ISO]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- lipid binding [IDA, ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein heterodimerization activity [TAS]
- receptor activity [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IMP, ISO]
- steroid hormone receptor activity [ISO, TAS]
- ubiquitin conjugating enzyme binding [ISO]
- DNA binding [IDA]
- MDM2/MDM4 family protein binding [ISO]
- NFAT protein binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- drug binding [ISO]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- lipid binding [IDA, ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein heterodimerization activity [TAS]
- receptor activity [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IMP, ISO]
- steroid hormone receptor activity [ISO, TAS]
- ubiquitin conjugating enzyme binding [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Gadd45 family proteins are coactivators of nuclear hormone receptors.
Gadd45 family genes encode nuclear acidic proteins composed of Gadd45, MyD118, and CR6. Sequence analysis showed that Gadd45 family proteins (Gadd45, MyD118, and CR6) contain LXXLL signature motifs considered necessary and sufficient for the binding of several coactivators to nuclear receptors. Interaction between Gadd45 or CR6 and RXR alpha was confirmed by a two-hybrid test in yeast. Results from a ... [more]
Biochem. Biophys. Res. Commun. May. 27, 2000; 272(1);193-8 [Pubmed: 10872826]
Throughput
- Low Throughput
Curated By
- BioGRID