BAIT

MPHOSPH6

MPP, MPP-6, MPP6
M-phase phosphoprotein 6
GO Process (1)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

SKIV2L2

Dob1, KIAA0052, Mtr4, fSAP118
superkiller viralicidic activity 2-like 2 (S. cerevisiae)
GO Process (2)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Mapping the Genetic Landscape of Human Cells.

Horlbeck MA, Xu A, Wang M, Bennett NK, Park CY, Bogdanoff D, Adamson B, Chow ED, Kampmann M, Peterson TR, Nakamura K, Fischbach MA, Weissman JS, Gilbert LA

Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]

Cell Jul. 17, 2018; (); [Pubmed: 30033366]

Quantitative Score

  • -3.037445915 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507)
  • cell type: k-562 cell (BTO:0000664)

Additional Notes

  • CRISPR GI screen
  • Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
  • Experimental Setup: Timecourse
  • GIST: A-phenotypic negative/positive genetic interaction
  • Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
  • K562 cell line Replicate Average GI score = -3.037445915
  • Library: CRISPRi v1
  • Significance Threshold: (positive genetic interaction) 3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MPHOSPH6 SKIV2L2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9973BioGRID
3177563
MPHOSPH6 SKIV2L2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High89.3581BioGRID
2941843
SKIV2L2 MPHOSPH6
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High-BioGRID
3443571

Curated By

  • BioGRID