NUP43
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cytokine-mediated signaling pathway [TAS]
- glucose transport [TAS]
- hexose transport [TAS]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- regulation of glucose transport [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
YTHDC1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Mapping the Genetic Landscape of Human Cells.
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]
Quantitative Score
- -5.707434708 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- cell type: k-562 cell (BTO:0000664)
Additional Notes
- CRISPR GI screen
- Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative/positive genetic interaction
- Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
- K562 cell line Replicate Average GI score = -5.707434708
- Library: CRISPRi v1
- Significance Threshold: (positive genetic interaction) 3
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| YTHDC1 NUP43 | Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis. | High | - | BioGRID | 3340205 | |
| NUP43 YTHDC1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3569906 |
Curated By
- BioGRID