BAIT
PSMD1
P112, Rpn2, S1
proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
GO Process (21)
GO Function (0)
GO Component (7)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- RNA metabolic process [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- apoptotic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- mitotic cell cycle [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- protein polyubiquitination [TAS]
- regulation of apoptotic process [TAS]
- regulation of cellular amino acid metabolic process [TAS]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- small molecule metabolic process [TAS]
- viral process [TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
VPS4A
SKD1, SKD1A, SKD2, VPS4, VPS4-1
vacuolar protein sorting 4 homolog A (S. cerevisiae)
GO Process (16)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- ATP catabolic process [NAS]
- abscission [IMP]
- cytokinesis [IDA]
- cytokinesis checkpoint [IMP]
- cytoplasmic microtubule organization [IBA]
- endosomal transport [IMP, TAS]
- membrane organization [TAS]
- negative regulation of cytokinesis [IMP]
- positive regulation of exosomal secretion [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
- ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway [IMP]
- vacuole organization [IBA]
- vesicle-mediated transport [IDA]
- viral budding via host ESCRT complex [IGI]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Mapping the Genetic Landscape of Human Cells.
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]
Cell Jul. 17, 2018; (); [Pubmed: 30033366]
Quantitative Score
- -3.481672497 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- cell type: k-562 cell (BTO:0000664)
Additional Notes
- CRISPR GI screen
- Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative/positive genetic interaction
- Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
- K562 cell line Replicate Average GI score = -3.481672497
- Library: CRISPRi v1
- Significance Threshold: (positive genetic interaction) 3
Curated By
- BioGRID