Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Mapping the Genetic Landscape of Human Cells.

Horlbeck MA, Xu A, Wang M, Bennett NK, Park CY, Bogdanoff D, Adamson B, Chow ED, Kampmann M, Peterson TR, Nakamura K, Fischbach MA, Weissman JS, Gilbert LA

Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]

Cell Jul. 17, 2018; (); [Pubmed: 30033366]

Quantitative Score

-4.606136407 [Confidence Score]

Throughput

High Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)
cell type: jurkat cell (BTO:0000661)
cell type: k-562 cell (BTO:0000664)

Additional Notes

CRISPR GI screen
Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
Experimental Setup: Timecourse
GIST: A-phenotypic negative/positive genetic interaction
Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
Jurkat cell line Replicate Average GI score = -3.865375512
K562 cell line Replicate Average GI score = -4.606136407
Library: CRISPRi v1
Significance Threshold: (positive genetic interaction) 3

Curated By

BioGRID

Interaction Summary

PTTG1

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase inhibitor activity [NAS]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]

Gene Ontology Cellular Component

UBA2