TAF1
Gene Ontology Biological Process
- DNA-templated transcription, initiation [ISS]
- RNA polymerase II transcriptional preinitiation complex assembly [ISS]
- cellular response to DNA damage stimulus [IC]
- gene expression [TAS]
- histone acetylation [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI]
- positive regulation of transcription initiation from RNA polymerase II promoter [ISS]
- protein autophosphorylation [IDA, TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- viral process [TAS]
Gene Ontology Molecular Function- TBP-class protein binding [IPI]
- histone acetyltransferase activity [IDA]
- lysine-acetylated histone binding [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- protein serine/threonine kinase activity [IDA]
- sequence-specific DNA binding [ISS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
- TBP-class protein binding [IPI]
- histone acetyltransferase activity [IDA]
- lysine-acetylated histone binding [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- protein serine/threonine kinase activity [IDA]
- sequence-specific DNA binding [ISS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
TAF1
Gene Ontology Biological Process
- DNA-templated transcription, initiation [ISS]
- RNA polymerase II transcriptional preinitiation complex assembly [ISS]
- cellular response to DNA damage stimulus [IC]
- gene expression [TAS]
- histone acetylation [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI]
- positive regulation of transcription initiation from RNA polymerase II promoter [ISS]
- protein autophosphorylation [IDA, TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- viral process [TAS]
Gene Ontology Molecular Function- TBP-class protein binding [IPI]
- histone acetyltransferase activity [IDA]
- lysine-acetylated histone binding [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- protein serine/threonine kinase activity [IDA]
- sequence-specific DNA binding [ISS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
- TBP-class protein binding [IPI]
- histone acetyltransferase activity [IDA]
- lysine-acetylated histone binding [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- protein serine/threonine kinase activity [IDA]
- sequence-specific DNA binding [ISS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Mapping the Genetic Landscape of Human Cells.
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]
Quantitative Score
- -9.066648993 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- cell type: jurkat cell (BTO:0000661)
Additional Notes
- CRISPR GI screen
- Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative/positive genetic interaction
- Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
- Jurkat cell line Replicate Average GI score = -9.066648993
- Library: CRISPRi v1
- Significance Threshold: (positive genetic interaction) 3
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
TAF1 TAF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID