Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

A DEAD-box RNA helicase Ddx54 protein in oligodendrocytes is indispensable for myelination in the central nervous system.

Zhan R, Yamamoto M, Ueki T, Yoshioka N, Tanaka K, Morisaki H, Seiwa C, Yamamoto Y, Kawano H, Tsuruo Y, Watanabe K, Asou H, Aiso S

We recently reported that a new monoclonal antibody, 4F2, which labels oligodendroglial lineage cells, recognizes a DEAD-box RNA helicase Ddx54 and that Ddx54 binds to myelin basic protein (MBP) in brain and cultured oligodendrocytes. To elucidate the biological function of Ddx54, we generated a recombinant adenovirus, Ad-shRNA:Ddx54, expressing a short hairpin RNA to silence endogenous Ddx54 protein. The virus was ... [more]

J. Neurosci. Res. Mar. 01, 2013; 91(3);335-48 [Pubmed: 23239230]

Throughput

  • Low Throughput

Ontology Terms

  • hek-293 cell (BTO:0000007)

Curated By

  • BioGRID