Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

CPEB2-eEF2 interaction impedes HIF-1α RNA translation.

Chen PJ, Huang YS

Translation of mRNA into protein proceeds in three phases: initiation, elongation, and termination. Regulated translation allows the prompt production of selective proteins in response to physiological needs and is often controlled by sequence-specific RNA-binding proteins that function at initiation. Whether the elongation phase of translation can be modulated individually by trans-acting factors to synthesize polypeptides at variable rates remains to ... [more]

EMBO J. Feb. 15, 2012; 31(4);959-71 [Pubmed: 22157746]

Throughput

  • Low Throughput

Curated By

  • BioGRID