Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Protein microarrays for the identification of praja1 e3 ubiquitin ligase substrates.

Loch CM, Eddins MJ, Strickler JE

Although they are the primary determinants of substrate specificity, few E3-substrate pairs have been positively identified, and few E3's profiled in a proteomic fashion. Praja1 is an E3 implicated in bone development and highly expressed in brain. Although it has been well studied relative to the majority of E3's, little is known concerning the repertoire of proteins it ubiquitylates. We ... [more]

Cell Biochem. Biophys. Jun. 01, 2011; 60(1);127-35 [Pubmed: 21461837]

Throughput

  • Low Throughput

Additional Notes

  • E2 interactor identified by ubiquitination assays.
  • Source of PRAJA1 not clear.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PJA1 UBE2D2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
UBE2D2 PJA1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
PJA1 UBE2D2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
3490331
UBE2D2 PJA1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID