PREY

ABL2

ABLL, ARG, RP11-177A2.3
ABL proto-oncogene 2, non-receptor tyrosine kinase
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Direct interaction of Jak1 and v-Abl is required for v-Abl-induced activation of STATs and proliferation.

Danial NN, Losman JA, Lu T, Yip N, Krishnan K, Krolewski J, Goff SP, Wang JY, Rothman PB

In Abelson murine leukemia virus (A-MuLV)-transformed cells, members of the Janus kinase (Jak) family of non-receptor tyrosine kinases and the signal transducers and activators of transcription (STAT) family of signaling proteins are constitutively activated. In these cells, the v-Abl oncoprotein and the Jak proteins physically associate. To define the molecular mechanism of constitutive Jak-STAT signaling in these cells, the functional ... [more]

Mol. Cell. Biol. Nov. 01, 1998; 18(11);6795-804 [Pubmed: 9774693]

Throughput

  • Low Throughput

Curated By

  • BioGRID