Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein.

Li H, Seth A

RING-finger proteins play crucial roles in ubiquitination events involved in diverse cellular processes including signal transduction, differentiation and apoptosis. Most of the RING-finger proteins have E3-ubiquitin ligase activity. RNF11 is a small RING-finger protein and harbors a RING-H2 domain and a PY motif that could facilitate protein:protein interaction(s) involved in oncogenesis. To isolate RNF11 protein partners and determine its role ... [more]

Oncogene Mar. 11, 2004; 23(10);1801-8 [Pubmed: 14755250]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

RNF11

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]protein binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

ZNF350

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding [IDA]RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Two-hybrid

Publication

An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein.

Throughput

Curated By

DNA binding [TAS]
protein binding [IPI]
ubiquitin-protein transferase activity [IDA]

DNA binding [IDA]
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding [IDA]
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
protein binding [IPI]