BAIT

CUT1

ptr4, SPCC5E4.04
separase/separin
GO Process (3)
GO Function (2)
GO Component (5)
Schizosaccharomyces pombe (972h)
PREY

STY1

phh1, spc1, SPAC24B11.06c
MAP kinase Sty1
GO Process (28)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Fission yeast MAP kinase is required for the increased securin-separase interaction that rescues separase mutants under stresses.

Kawasaki Y, Nagao K, Nakamura T, Yanagida M

Sister chromatid separation requires two steps of proteolysis. Securin, the chaperon and inhibitor of separase, is destructed in anaphase after polyubiquitination, and resulting activated separase cleaves the cohesin subunit Scc1/Rad21. Fission yeast securin/Cut2 and separase/Cut1 that form the complex are essential for viability and a number of temperature-sensitive (ts) mutants have been isolated. We here report that the stresses such ... [more]

Cell Cycle Aug. 01, 2006; 5(16);1831-9 [Pubmed: 16861909]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: heat sensitivity (APO:0000147)
  • phenotype: inviable (APO:0000112)

Curated By

  • BioGRID