Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Neddylation requires glycyl-tRNA synthetase to protect activated E2.

Mo Z, Zhang Q, Liu Z, Lauer J, Shi Y, Sun L, Griffin PR, Yang XL

Neddylation is a post-translational modification that controls the cell cycle and proliferation by conjugating the ubiquitin-like protein NEDD8 to specific targets. Here we report that glycyl-tRNA synthetase (GlyRS), an essential enzyme in protein synthesis, also plays a critical role in neddylation. In human cells, knockdown of GlyRS, but not knockdown of a different tRNA synthetase, decreased the global level of ... [more]

Nat. Struct. Mol. Biol. Dec. 01, 2015; 23(8);730-7 [Pubmed: 27348078]

Throughput

  • Low Throughput

Additional Notes

  • #LPPI

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GARS NEDD8
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
2461059
NEDD8 GARS
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
2461064

Curated By

  • BioGRID