PREY

STUB1

CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1, LA16c-313D11.6
STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
GO Process (15)
GO Function (15)
GO Component (10)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Wang X, Cao W, Zhang J, Yan M, Xu Q, Wu X, Wan L, Zhang Z, Zhang C, Qin X, Xiao M, Ye D, Liu Y, Han Z, Wang S, Mao L, Wei W, Chen W

Enhancer of zeste homolog 2 (EZH2) has been characterized as a critical oncogene and a promising drug target in human malignant tumors. The current EZH2 inhibitors strongly suppress the enhanced enzymatic function of mutant EZH2 in some lymphomas. However, the recent identification of a PRC2- and methyltransferase-independent role of EZH2 indicates that a complete suppression of all oncogenic functions of ... [more]

EMBO J. Dec. 02, 2016; 36(9);1243-1260 [Pubmed: 28320739]

Throughput

  • Low Throughput

Curated By

  • BioGRID