BAIT

URM1

ubiquitin-related modifier URM1, S000007535, YIL008W
Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
GO Process (6)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

TEF1

translation elongation factor EF-1 alpha, eEF1A, EF-1 alpha, L000002277, YPR080W
Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF1 has a paralog, TEF2, that arose from the whole genome duplication
GO Process (2)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Collaboration of tRNA modifications and elongation factor eEF1A in decoding and nonsense suppression.

Klassen R, Schaffrath R

Transfer RNA (tRNA) from all domains of life contains multiple modified nucleosides, the functions of which remain incompletely understood. Genetic interactions between tRNA modification genes in Saccharomyces cerevisiae suggest that different tRNA modifications collaborate to maintain translational efficiency. Here we characterize such collaborative functions in the ochre suppressor tRNA SUP4. We quantified ochre read-through efficiency in mutants lacking either of ... [more]

Sci Rep Aug. 24, 2018; 8(1);12749 [Pubmed: 30143741]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • genetic complex
  • there is a clear improvement of growth of the deg1/urm1 and deg1/elp3 mutants, but not of the respective single mutants, upon overexpression of eEF1A
  • there is an improvement of growth of the tcd1/urm1 double mutant upon overexpression of eEF1A

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TEF1 URM1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1574BioGRID
2195548

Curated By

  • BioGRID