A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.

Matsumura T, Yuasa T, Hayashi T, Obara T, Kimata Y, Yanagida M

BACKGROUND: The fission yeast Schizosaccharomyces pombe separase/Cut1 and securin/Cut2 are required for anaphase-specific activation of proteolysis that leads to proper sister chromatid separation. We intended to identify ts (temperature sensitive) strains whose growth was inhibited by multicopy plasmid pCUT1 or pCUT2 at the permissive temperature. RESULTS: After a one-by-one transformation of 1015 randomly isolated ts strains, 18 transformants that retarded ... [more]

Genes Cells Apr. 01, 2003; 8(4);341-55 [Pubmed: 12653962]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CUT9 CUT2	Dosage Growth Defect Dosage Growth Defect A genetic interaction is inferred when over expression or increased dosage of one gene causes a growth defect in a strain that is mutated or deleted for another gene.	Matsumura T (2003)	Low	-	BioGRID	246259
CUT9 CUT2	Dosage Lethality Dosage Lethality A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.	Yuasa T (2004)	Low	-	BioGRID	247888

Curated By

BioGRID

Interaction Summary

CUT2

Gene Ontology Biological Process

Gene Ontology Molecular Function

peptidase inhibitor activity [IMP]

Gene Ontology Cellular Component

CUT9