A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.

Matsumura T, Yuasa T, Hayashi T, Obara T, Kimata Y, Yanagida M

BACKGROUND: The fission yeast Schizosaccharomyces pombe separase/Cut1 and securin/Cut2 are required for anaphase-specific activation of proteolysis that leads to proper sister chromatid separation. We intended to identify ts (temperature sensitive) strains whose growth was inhibited by multicopy plasmid pCUT1 or pCUT2 at the permissive temperature. RESULTS: After a one-by-one transformation of 1015 randomly isolated ts strains, 18 transformants that retarded ... [more]

Genes Cells Apr. 01, 2003; 8(4);341-55 [Pubmed: 12653962]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Curated By

BioGRID

Interaction Summary

CUT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

endopeptidase activity [IMP]protein binding [IPI]

Gene Ontology Cellular Component

CUT4