BAIT
KDM2B
CXXC2, FBXL10, Fbl10, JHDM1B, PCCX2
lysine (K)-specific demethylase 2B
GO Process (15)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- embryonic camera-type eye morphogenesis [ISS]
- forebrain development [ISS]
- fourth ventricle development [ISS]
- hindbrain development [ISS]
- histone H2A monoubiquitination [IDA]
- histone demethylation [TAS]
- initiation of neural tube closure [ISS]
- lateral ventricle development [ISS]
- midbrain development [ISS]
- midbrain-hindbrain boundary morphogenesis [ISS]
- negative regulation of neural precursor cell proliferation [ISS]
- negative regulation of neuron apoptotic process [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- spermatogenesis [ISS]
- third ventricle development [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRMT5
HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs
protein arginine methyltransferase 5
GO Process (13)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- cell proliferation [TAS]
- circadian regulation of gene expression [ISS]
- endothelial cell activation [IMP]
- gene expression [TAS]
- histone H4-R3 methylation [ISS, NAS]
- ncRNA metabolic process [TAS]
- peptidyl-arginine N-methylation [IDA]
- peptidyl-arginine methylation [IMP]
- peptidyl-arginine methylation, to symmetrical-dimethyl arginine [IMP]
- regulation of mitosis [TAS]
- regulation of transcription, DNA-templated [IBA]
- spliceosomal snRNP assembly [IMP, TAS]
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
The orchestrated action of genes controls complex biological phenotypes, yet the systematic discovery of gene and drug combinations that modulate these phenotypes in human cells is labor intensive and challenging to scale. Here, we created a platform for the massively parallel screening of barcoded combinatorial gene perturbations in human cells and translated these hits into effective drug combinations. This technology ... [more]
Proc. Natl. Acad. Sci. U.S.A. Mar. 01, 2016; 113(9);2544-9 [Pubmed: 26864203]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507) [ovcar-8/adr cell (BTO:0004189)]
Additional Notes
- CRISPR GI screen
- Cell Line: OVCAR-8/ADR cell BTO:0004189
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Identified 61 gene combinations as top hits that resulted in antiproliferative effects (log2 ratio < -0.90) in both biological replicates (Q-value < 0.01).
- Library: Gecko v2
- Pooled screen using a GeCKOv2 CRISPR library with barcode abundances compared between day 15 and day 20 groups.
- Significance Threshold: log2 ratio< -0.90; Q-value< 0.01
- Used CombiGEM-CRISPR technology to identify gene pairs that inhibited ovarian cancer cell growth (OVCAR8-ADR cells).
Curated By
- BioGRID