BAIT

DDX39B

BAT1, D6S81E, UAP56, DAAP-97M17.4
DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
Homo sapiens
PREY

SPTBN1

ELF, HEL102, SPTB2, betaSpII
spectrin, beta, non-erythrocytic 1
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in β-Catenin-Active Cancers.

Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe J, Lage K, Boehm JS, Hahn WC

Genome-scale expression studies and comprehensive loss-of-function genetic screens have focused almost exclusively on the highest confidence candidate genes. Here, we describe a strategy for characterizing the lower confidence candidates identified by such approaches. We interrogated 177 genes that we classified as essential for the proliferation of cancer cells exhibiting constitutive β-catenin activity and integrated data for each of the candidates, ... [more]

Cell Syst Dec. 28, 2015; 3(3);302-316.e4 [Pubmed: 27684187]

Throughput

  • High Throughput

Additional Notes

  • High-credibility protein interactions were identified using draft-PPI and a method for protein interaction credibility scoring (ICS).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DDX39B SPTBN1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID